![]() When coupled to a confocal microscope 8, Brillouin microscopy can achieve three-dimensional (3D) diffraction-limited resolution. By analyzing the frequency shift ( v B) and linewidth (Γ B) of the light that is inelastically scattered from gigahertz-frequency longitudinal acoustic vibrations (phonons) in the sample, one can deduce the elastic and viscous properties of the sample, respectively. Recently, Brillouin spectroscopy has emerged as a non-destructive, label- and contact-free technique that can assess the viscoelastic properties of biological samples via photon–phonon scattering interactions 5, 6, 7. ![]() Although molecular components of cells can routinely be visualized with fluorescence microscopy in biology, assessment of the mechanical, that is, elastic and viscous, properties of living cells with similar spatiotemporal resolution is more challenging given the intrinsic limitations of current biophysical techniques 2, 3, 4. The fields of mechanobiology and physical biology have grown quickly in the past decade, revealing that cell and tissue mechanics play an integral role in determining biological function 1.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |